Issue: 2025, Vol. 30, No. 2
THE IMPORTANCE OF GENE MUTATIONS IN THE DEVELOPMENT OF METABOLIC SYNDROME IN PATIENTS WITH CHRONIC HEPATITIS C
- Keywords
- chronic hepatitis C, metabolic syndrome, lipid metabolism, liver fibrosis, LPL gene, FTO gene
- Abstarct
- ABSTRACT Objective – to identify clinical, laboratory and genetic features of chronic hepatitis C (CHC) patients with metabolic disorders. Material and Methods. 201 patients aged from 18 to 60 years old were examined in the State Budgetary Institution ‘Regional Infectious Clinical Hospital’ of Yaroslavl region. Anamnestic data were collected, anthropometric measurements being performed. The diagnosis of hepatitis C was verified through the detection of antibody spectrum (a-cor, a-NS3, a-NS4, a-NS-5) in blood serum by enzyme-linked immunosorbent assay (ELISA) and HCV RNA by polymerase chain reaction (PCR). The clinical diagnostic laboratory of the Institute of Pharmacy of FSBI HE Yaroslavl State Medical University of the Ministry of Public Health of Russia carried out the molecular genetic study. Gene polymorphisms were tested by real-time PCR on iCycler iQ5 (BioRad) with SNP-express-RV reagent kit, polymorphisms of the genes APOE Leu- 28Pro, LPL S447X and FTO A23525T were studied. All patients underwent ultrasound examination of the liver, FibroScan 502 device helping to reveal the hepatic fibrosis (HF). Results and Discussion. The patients were divided into groups according to the inclusion criteria: the first (main) group involved 161 patients with CHC with metabolic syndrome (MS), 40 patients with CHC without MS being in the second, comparison group. The availability of LPL S447X and FTO A23525T gene mutations in patients resulted in high degree of hepatitis activity, stages of FP F3-F4, dyslipidaemia and severity of MS components, direct correlations between them being revealed. Conclusion. Metabolic disorders significantly worsen the course of HCV infection and are significantly more often associated with the progression of FP.
Vestnik_2_30-12-18