Issue: 2012, Vol. 17, No. 2

Safonova M. A., Kuznetsov Олег Ювенальевич

IMPROVEMENT OF THE TECHNIQUE OF BACTERIA MICROCULTIVATION IN THE EVALUATION OF THEIR POPULATIONS DEVELOPMENT PECULIARITIES

Keywords

microcultivation, quorum-sensing, chambers of stationary and fl owing types, generation time, Shigella fl exneri

Abstarct

The information concerning the possibility of using of microchambers of stationary and fl owing types while microcultivation of bacterial cells was presented. Formalized indices of the development of Shigella flexneri S-50 and Shigella flexneri Rd were studied. The length and the diameter at the beginning and at the end of cellular cycle were determined so as the time of the second generation production. It was stated that it was useful to take into account the production time for the fi rst two generations as the mostly optimal and integral index for the evaluation of physiological status of bacterial population cells. Special attention should be paid to the adaptive behavior of the second generation cells which was refl ected by the time of production.