Issue: 2023, Vol. 28, No. 4

А. М. Karachenova, Е. N. Romanova, А. А. Dutova

GENETIC PREDICTORS OF THE DEVELOPMENT AND SEVERITY OF COVID-19-ASSOCIATED LUNG LESION

Keywords

COVID-19-associated lung lesion, gene polymorphism, flu А/Н1N1/09.

Abstarct

Оbjective – to study the polymorphism of cytokine genes (tumor necrosis factor – TNFG308A, interleukins – IL10C592A, IL10G1082A), gene of regulatory molecule of inflammation (CD14C159T) in pa- tients after Covid-19-associated lung lesion. Material and methods. 156 patients after suffering pneumonia on the background of COVID-19 in one month after discharge from the hospital were enrolled in the study. All patients were divided into groups in dependence on the level of lung lesion upon the results of computer tomography (КТ): the first group (n = 74) – КТ-1, 2; the second group (n = 82) – КТ-3, 4. 56 healthy persons composed the control group. Molecular genetic studies of cytokine genes (TNFG308A, IL 10 C592A, IL 10 G1082A), gene of regulatory molecule of inflammation (CD14C159T) were performed by polymerase chain reaction (PCR) with allele-specific primers (LEGENDplexTM). Results and discussion. In the study of TNFG308A gene polymorphism the authors revealed 1,3 times more frequent G allele carriage (р < 0,001, odds ratio = 3,6) in comparison with control group, IL 10 G1082A receptor gene – more frequent (6 times) G allele carriage in patients from the second group (р < 0,001). G/G genotype was 7,5 times more common (р < 0,001) in the patients from the second group in comparison with the patients from the first group. The analysis of IL10C592A gene polymorphism demonstrated more frequent (3,4 times) occurrence of C allele (р < 0,001; odds ratio = 13), and also more frequent carriage of C/C genotype (4,5 times) in the patients from the second group in comparison with the persons from the control group (р < 0,001). The studying of CD14C159T receptor gene demonstrated more frequent (1,4 times) allele С carriage in the patients in comparison with healthy persons from the control group (р< 0,001; odds ratio = 2,4). Conclusion. The performed study revealed the interrelation between the development and severity of COVID-19-associated lung lesion and certain polymorphisms of studied cytokines.

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