Issue: 2022, Vol. 27, No. 2
INFLUENCE OF PULCE-THERAPY ON THE CONTENT OF TUMOR NECROSIS FACTOR-α, INTERLEUKIN-4 AND PLATELET VASCULAR HOMEOSTASIS IN SYSTEMIC LUPUS ERYTHEMATOSUS
- Keywords
- Tumor necrosis factor-α, interleukin-4, Willebrand factor antigen, platelet aggregation, methylprednisolone, hemostasis, systemic lupus erythematosus
- Abstarct
- objective – to study the dynamics of tumor necrosis factor-α level (TNF-α), interleukin-4 (IL- 4), Willebrand factor antigen (WF:Аg), thrombocyte aggregation in systemic lupus erythematosus (SLE) after intensive therapy by high doses of glucocorticoids. Material and methods. 13 patients with SLE were examined. Average age – 35,5 years, average duration of the disease – 10 years. 9 patients had SLEDAI2К index amounting to 4–12 points, 4 patients had this index parameters higher than 12 points. Pulse-therapy by methylprednisolone (1000 mg intravenously) per day within three days was administered to the patients. Hemostasiological and immunological examination was conducted before pulse-therapy and after its end. The determination of the concentration of TNF-α, IL-4, WF:Аg in blood serum was made by enzyme immunoassay (EIА). The status of platelet link of homeostasis was studied by BIOLA230LA aggregometer. The data were presented in the form of Ме (25%; 75%). Results and discussion. Initial increase of TNF-α, IL-4, С-reactive protein (СRP), WF:Аg, spontaneous platelet aggregation parameters was marked. WF:Аg level decreased from 1,38 ± 0,81 to 1,15 ± 0,18 Ме/ml (р < 0,05). After pulse-therapy TNF-α concentration diminished from 13,9 (9,4–20,9) tо 11,6 (4,4–16,0) pg/ml (р < 0,05). IL-4 level and spontaneous platelet aggregation parameters after pulse-therapy did not differ from the analogous ones in the control group. Conclusion. «Classic» three day pulse-therapy by methylprednisolone resulted in the alterations of both immunological parameters and separate indices of platelet-vascular hemostasis.
Vestnik_2_2022-26-30