Issue: 2021, Vol. 26, No. 2

V. I. Emanuylov, N. V. Emanuylova, А. V. Arshinov

INTERLEUKIN-4 CONTENT IN SYSTEMIC LUPUS ERYTHEMATOSUS: INTERACTION WITH AUTOIMMUNE INFLAMMATION AND HEMOCOAGULATION DISORDERS

Keywords

IL-4, hemostasis, systemic erythematosus

Abstarct

Objective – to study interleukin-4 content (IL-4) in patients with systemic lupus erythematosus (SLE) and to estimate its interaction with some clinical laboratory hemocoagulation parameters depending on antiphospholipid syndrome presence (APS). Material and methods. 60 patients with SLE, average age – 33,5 years, average duration of the disease – 10 years – were examined. Patients with subacute course of the disease and moderate degree of the process activity predominated. IL-4 content in blood serum was performed by the ELISA method with the application of commercial reagents «Vectorbest » (Russia). Antibodies to ds-DNA were revealed by solid-phase ELISA method with the application of commercial sets of SigmaChemikal (USA). Von Willebrandt factor antigen (vWFAg) was determined in blood serum by ELISA method (TechnozymvWF: AgElisa, Austria). The state of platelet link of hemostasis was studied by aggregometer BIOLA230LA, coagulation parameters – by coagulometer SOLAR. Fibrinogen concentration, level of fibrin-monomer complexes, indicators of spontaneous fibrinolysis and antithrombin III activity were determined. Results and discussion. IL-4 content in patients from the control group was amounting to 7,2 (3–12,5) pg/ml, in patients with SLE – 14,25 (7–25,5) pg/ml (р < 0,05). IL-4 level in patients with APS was higher (15,6 (6,9–25,6) pg/ml) than in patients without APS (13,1 (7,1–23,9) pg/ml). In the common group of patients with SLE there were demonstrated direct correlations of IL-4 level with anti-dsDNA (r = 0,64; p < 0,05), in the group of patients with APS – with platelet spontaneous aggregation index (r = 0,67; p < 0,05), with clinical activity index by SLEDAI2К scale (r = 0,71; p < 0,05) and such clinical parameters as vascular butterfly (r = 0,7; p < 0,05), thrombocytopenia (r = 0,7; p < 0,05) and thrombosis presence in the anamnesis (r = 0,76; p < 0,05). Conclusions. The interpretation of the obtained results of the investigation in total with clinical laboratory parameters is not obvious. These results allowed to propose anti-inflammatory effect and activation of platelet link of hemostasis at least in patients with SLE and APS presence which was induced by IL-4.

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• Vestnik 2 2021 sbornik-25-29