Issue: 2018, Vol. 23, No. 3

Scherbakova M. L., Kononova A. O., Ilyin M. V., Lapkina N. A., Baranov A. A.

HORMONAL IMBALANCE AND NEUTROPHIL FUNCTIONAL ACTIVITY IN ANKYLOSING SPONDILITIS

Keywords

ankylosing spondylitis, neutrophil, estradiol, testosterone

Abstarct

Objective - to study the correlation between sex hormones concentrations and neutrophil redox regulation in patients with ankylosing spondylitis. Material and methods. 82 patients with ankylosing spondylitis were examined. Authors used ficoll-urografin double density gradient to isolate neutrophils. The chemiluminescent method was used to evaluate neutrophil-ROS-generating capacity. Method of immunoferment detection was used to determine estradiol and testosterone concentrations. Results. The increase of neutrophil free radicals production associated with the increase of superoxide anion generating capacity and decrease of neutrophil-ROS-generating capacity were observed in ankylosing spondylitis. These changes of cell biocidity were associated with the decrease of estradiol concentration, the increase of testosterone level and estrogenic ratio. The direct correlation between estradiol level and neutrophil-ROS-generating capacity was detected. The strong correlation between testosterone concentration, neutrophil-ROS-generating capacity and superoxide radical level was revealed. ROS-generating potential of neutrophils was directly connected to testosterone concentrations. Conclusion. The increase of neutrophil redox regulation which was followed by the heightening of superoxide anion production capacity and decrease of neutrophil-ROS-generating capacity against the background of the decrease of estradiol concentration, the increase of testosterone level and estrogenic coefficient which were correlated to the indices of neutrophil functional activity were observed in ankylosing spondylitis.

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