Issue: 2023, Vol. 28, No. 4

Е. G. Оrlova, О. А. Loginova, О. L. Gorbunova, N. V. Кarimova, N. P. Loginova, S. V. Shirshev

METABOLIC ACTIVITY OF VARIOUS SUBPOPULATIONS OF NATURAL KILLERS OF PERIPHERAL BLOOD IN PHYSIOLOGICALLY OCCURING PREGNANCY

Keywords

natural killers, pregnancy, Glut-1, мitochondria mass.

Abstarct

Objective – to estimate metabolic activity of various subpopulations of natural killers (NK-cells) of peripheral blood in the first and third trimesters of physiologically occurring pregnancy (POP). Мaterial and methods. Healthy women in the first or in the third trimester of POP were enrolled in the study. Comparison group was formed of conditionally healthy women in the follicular phase of the menstrual cycle. Peripheral blood was the object of the research. Metabolic activity was evaluated by expression of glucose transporter protein Glut-1 and activation of the mitochondrial probe MitoSpyGreen, reflected the mass and the volume of mitochondrial compartment, by flow cytofluorometry method on regulatory (CD56 bright CD16 - ), cytotoxic (CD56 dim CD16 + ), minor cytotoxic (CD56 - CD16 hi )NK-cells. Results and discussion. In non-pregnant women the quantity of metabolically activated cells expressing Glut-1 and having larger mass and volume of mitochondria (Mito + ), was significantly higher in the subpopulation of cytotoxic CD56 - CD16 hi NK-cells than among regulatory CD56 bright CD16 - and cyto- toxic CD56 dim CD16 + NK-cells. In the first trimester of pregnancy the level of Glut-1 + Mito + regulatory CD- 56 bright CD16 - and cytotoxic CD56 dim CD16 + NK-cells significantly increased in comparison with the same level in non-pregnant women, then how the percentage of cytotoxic Glut-1 + Mito + CD56 - CD16 hi NK-cells remained high but did not change. In the III trimester the quantity of Glut-1 + Mito + regulatory CD56 bright CD16 - NK-cells remained higher than in non-pregnant women. The quantity of cytotoxic Glut-1 + Mito + CD56 dim CD16 + NK-cells decreased in comparison with the same parameter in non-pregnant women, and Glut-1 + Mito + CD56 - CD16 hi NK-cells in relation to the first trimester. Conclusion. The quantity of metabolically cytotoxic and regulatory NK-cells of peripheral blood increased in POP.

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• Vestnik_4_2023-23-28