Issue: 2017, Vol. 22, No. 3

Fetisova I. N., Chasha T. V., Mezhinsky S. S., Ratnikova S. Yu., Fetisov N. S.

GENE POLYMORPHISM OF HLA SYSTEM OF II CLASS IN PREMATURE NEWBORNS WITH BRONCHOPULMONARY DYSPLASIA

Keywords

gene polymorphism, deeply premature infants, bronchopulmonary dysplasia, risk factors, main complex of histocompatibility, genetic factors

Abstarct

Objective - to examine the peculiarities of gene polymorphism of HLA system of II class (DRBI, DQA1, DQB1) in deeply premature newborns with body mass in birth lower than 1500 g with bronchopulmonary dysplasia (BPD) and to determine the general risk factors for this diseases formation.Material and methods. Complex examination was performed in 97 infants in the Ivanovo Research Institute of Maternity and Childhood; infants with formed BPD were enrolled in the first group (n = 50), infants without BPD (n = 47) were enrolled into the second group. Genome DNA was isolated from lymphocytes of venous blood and epithelial cell of buccal brushings, test of gene of HLA system of II class were performed by classic polymerase chain reaction. Results. The increase of prevalence of DRB1 17, DQB1 0201 alleles and combinations of DRB1 17 and DQB1 0201, DRB1 17 and DQB1 0501 were revealed; this fact was allowed to testify to unfavorable additive effect of the above mentioned alleles. There was no combination of DQA1 0301, DQB1 0501 alleles in premature newborns with BPD; in the group of recovered infants it was observed in 13,3% cases; this fact allowed to suggest that this peculiarity might act as genetic factor which could prevent BPD development. The differences between the main and the control groups in the prevalence DRB1-DQA1-DQB1 haplotype were revealed.Conclusions. The presence of DRB1 17, DQA1 0501 and DQB1 0201 alleles, combined presence of DRB1 17 and DQB1 0201, DRB1 17 and DQB1 0501 also the presence of DRB1-DQA1-DQB1 01-0101-0501 haplotype in the genotype of the preterm infant was proved to be the risk factor for bronchopulmonary dysplasia development and was allowed to be used as the genetic marker for the studied pathology development.

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