Issue: 2016, Vol. 21, No. 2
SPLEEN MORPHOFUNCTIONAL CHARACTERISTICS IN EXPERIMENTAL HYPERKALEMIA IN RATS
- Keywords
- spleen, calcium medicaments, hyperkalemia, morphofunctional characteristics, lymphoid nodes, red pulp
- Abstarct
- Objective - morphological analysis of reactive alterations of spleen morphofunctional zones in rats in response to enrichment of water ration with calcium chloride. Material and methods . 64 rats with body mass amounting to 150-200 g were examined. The animals of the 1 control group (n = 32) were given table water, the animals of the 2 experimental group (n = 32) were given table water with calcium chloride within two months. Morphological investigation included the definition of the quantity and the square of primary and secondary spleen lymphoid nodes, the measurement of total area of white and red pulp and their correlation. Morphometric borders of lymphoid nodes were determined by sigmal method; calcium content in blood serum was defined by colorimetric method with o-crezolphtaleincomplexon use. Results . On the background of prolonged impact of calcium with table water there were marked hyperplastic changes of white spleen pulp in laboratory rats: lymphoid nodes were inclined to confluence and enlargement, quantity and square of primary and secondary lymphoid nodes at all surface of sections were increased. Quantitative prevalence of primary (66,6%) lymphoid nodes beyond the secondary ones was demonstrated in experimental group; their quantity in control group was comparatively equal. The number of structural components of white spleen pulp with reproduction center was reduced by 9%; the most part of them was presented by lymphoid nodes with undeveloped germinal center (30,6%). The enrichment of table water by calcium was accompanied by the alteration of spleen functional zones ratio: significant increase of white pulp square and decrease of red pulp square. The trustworthy rise in general calcium content in blood serum by 0,34 ± 0,08 mmol/l (11,5 + 2,61% from control parameters) was showed in experimental animals. Conclusions . Hyperplastic disorders of spleen white pulp were noted in experimental rats blood in general calcium concentration heightening; it was accompanied by functional zones ratio alteration.
Мельникова